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KMID : 0380019940090030287
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 3 p.287 ~ p.292
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Effects of cultivation Condition on Growth of the Hydrogen Sulfide-Degradating Thiobacillus sp. IW. Isolated from Waste Coal Mine Water
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Abstract
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A bacterium isolated from waste coal mine water around Hawsun had an ability for the degradation of hydrogen sulfide. The isolate was identified as Thiobacillus sp. 1W. on the basis of its morphological, physiological and chemotaxonomical characteristics. The optimum pH and temperature were 7 and 30 C, respectively. Growth occured in a pH range of 3 to 9. Due to the sulfate accumulated in liquid medium, the pH decreased. As a consequence the cell growth was inhibited. Potasium nitrate and glutamic acid were utilized as a nitrogen source but urea and ammonium chloride not consumed. Denitrif ication occured in a basal medium containing the glucose but did not in a basal medium containing the malate. The maximun specific growth rate of cell was 0.78h-´ and generation time was 0.9 hour. The cell productivity was 6.25mg/l ? h and the isolate grew logarithmically up to 18 hour. These results indicate that the isolate can be a suitable bacterium responsible for degradation of hydrogen sulfide as malodorous compounds.
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